Mutational Analysis Using Enriched PCR and Cycle Sequencing
نویسندگان
چکیده
منابع مشابه
Mutational analysis using enriched PCR and cycle sequencing.
During the course of our research on chemically induced mutational spectra in mouse skin and liver tumors, we found the need to develop a sensitive method that would screen out nonmutated genes, related genes and pseudogenes. Although cold single-strand conformation polymorphism methods that used intron-specific primers existed, these methods were only sensitive to the point of 1 mutant in 33 n...
متن کاملRapid sequencing of unpurified PCR products by thermal asymmetric PCR cycle sequencing using unlabeled sequencing primers.
Numerous methods have been developed for direct sequencing of single or double-stranded DNA amplified by PCR, and most require template purification steps prior to sequencing to remove excess unincorporated primers and dNTPs. Purification methods can be cumbersome, laborious and time consuming, or yield low DNA recoveries. Strategies for direct sequencing of doublestranded DNA products without ...
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MATERIALS 5x Cycle-sequencing buffer AmpliTaq CS or other exonuclease-deficient versions of Taq DNA polymerase (5 units/μl) EDTA (0.05 M, pH 8.0) Formamide loading buffer Oligonucleotide Primers, radiolabeled at the 5' terminus with 33P or 32P To prepare end-labeled primers, please see Phosphorylating the 5' Termini of Oligonucleotides. Template DNAs Plasmids, cosmids, bacteriophage , and bacte...
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In this study, genetic differences and phylogenic relationships among six Mugilidae species (Mugil cephalus, M. capito, Liza subviridis, L. saliens, L. aurata, Valamugil buchanani) were determined using PCR-sequencing. M. cephalus, L. subviridis, and V. buchanani from the Persian Gulf and Oman Sea, and L. aurata and L. saliens from the Caspian Sea were col-lected. Samples of an imported, Egypt...
متن کاملA phylogeny analysis on six mullet species (Teleosti: Mugillidae) using PCR-sequencing method
In this study, genetic differences and phylogenic relationships among six Mugilidae species (Mugil cephalus, M. capito, Liza subviridis, L. saliens, L. aurata, Valamugil buchanani) were determined using PCR-sequencing. M. cephalus, L. subviridis, and V. buchanani from the Persian Gulf and Oman Sea, and L. aurata and L. saliens from the Caspian Sea were col-lected. Samples of an imported, Egypt...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1998
ISSN: 0736-6205,1940-9818
DOI: 10.2144/98246cr02